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OBJECTIVE: To study the compatibility of inner surface of anatomy bottle and Caffeine citrate injection.METHODS: After treated with high temperature (60 ℃, 10 days), light (5 000 Lx, 10 days), freezing and thawing (2 days for freezing at -18 ℃ and 2 days for thawing at 40 ℃ as a circulation, and repeat for 3 times), sterilizing (121 ℃, 2 h), Caffeine citrate injection packed in anatomy bottle was tested in respects of pH value, insoluble particle, glass stripping tendency before test and under the above conditions. The contents of arsenic, cadmium, lead, cobalt, manganese, vanadium, copper, lithium, barium, chromium, antimony, aluminum, boron, calcium, iron, potassium, magnesium, silicon and titanium that may dissolve by inner surface of anatomy bottle were tested by ICP method, and compared with analysis evaluation threshold (AET) of each element. RESULTS: Before the test, under high temperature, light, freezing and thawing, sterilization, and pH values of Caffeine citrate injection installed with ampoule were 4. 58, 4. 60, 4. 61, 4. 58, 4. 58. The insoluble particles were all in line with 0903 provisions stated in (four) general provisions of Chinese Pharmacopoeia (2015 edition). The inner surface of ampoule bottle glass had no stripping tendency. The arsenic, cadmium, lead, cobalt, manganese, vanadium, copper, lithium, barium, chromium, antimony, iron, magnesium and titanium were not detected in caffeine citrate injection, and the contents of aluminum, boron, calcium, potassium and silicon were far below its AET value. CONCLUSIONS: Caffeine citrate injection has no effect on inner surface of ampoule bottle.
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Objective To investigate the curative effect of microwave ablation for non-small cell lung cancer (NSCLC) patients,and to analyze the serum concentration changes of vascular endothelial growth factor (VEGF),arginase-1 (Arg-1),inducible nitric oxide synthase (iNOS) before and after microwave ablation and their correlations.Methods A total of 30 cases of healthy people (control group) and 30 cases of advanced NSCLC (test group) were selected.The serum concentrations of VEGF,Arg-1 and iNOS in control group and test group (before microwave ablation,the first postoperative day,the third postoperative day and the first postoperative month) were measured by enzyme-linked immunosorbent assay (ELISA).Results The effective rate of microwave ablation for advanced NSCLC was 33.3％ (10/30),and the disease control rate was 70.0％ (21/30).The concentrations of VEGF,Arg-1 and iNOS in test group before microwave ablation were (816.56 ± 13.26)pg/ml,(5.17±0.20) ng/ml and (544.18 ± 13.93)pg/ml,which were higher than those in control group (93.43 ± 9.93) pg/ml,(1.08 ± 0.05) ng/ml and (8.08 ± 0.33) pg/ml,and the differences were statistically significant (t =239.093,P ＜ 0.001;t =110.359,P ＜ 0.001;t =210.792,P ＜ 0.001).The concentrations of VEGF were (708.41 ± 10.49) pg/ml,(592.63 ± 7.25) pg/ml and (521.91 ± 8.32) pg/ml on the first day,third day and 1 month after microwave ablation in patients with advanced NSCLC,which were significantly lower than those before treatment (all P ＜ 0.05).The homologous concentrations of Arg-1 were (5.95 ± 0.10) ng/ml,(7.02 ± 0.13) ng/ml and (7.67 ± 0.92) ng/ml,which were significantly higher than those before treatment (all P ＜ 0.05).The homologous concentrations of iNOS were (453.01 ± 9.48) pg/ml,(393.21 ± 9.42) pg/ml and (352.60 ± 8.31) pg/ml,which were significantly lower than those before treatment (all P ＜ 0.05).The expression of iNOS was positively related with VEGF in NSCLC patients before treatment (r =0.379,P =0.039),and the expression of Arg-1 was negatively related with VEGF (r =-0.556,P =0.001).However,the expression of iNOS was not associated with Arg-1 (r =-0.238,P =0.205).Conclusion Microwave ablation is effective for local therapy of NSCLC,which can directly kill cancer cells,and affect the levels of VEGF,Arg-1 and iNOS.VEGF has certain correlation with iNOS and Arg-1,but there was no correlation between iNOS and Arg-1.Microwave ablation can change the tumor microenvironment in a certain extent,and stimulate the body to produce anti-tumor immunity.
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Objective To investigate the curative effect of microwave ablation for non-small cell lung cancer (NSCLC) patients,and to analyze the serum concentration changes of vascular endothelial growth factor (VEGF),arginase-1 (Arg-1),inducible nitric oxide synthase (iNOS) before and after microwave ablation and their correlations.Methods A total of 30 cases of healthy people (control group) and 30 cases of advanced NSCLC (test group) were selected.The serum concentrations of VEGF,Arg-1 and iNOS in control group and test group (before microwave ablation,the first postoperative day,the third postoperative day and the first postoperative month) were measured by enzyme-linked immunosorbent assay (ELISA).Results The effective rate of microwave ablation for advanced NSCLC was 33.3％ (10/30),and the disease control rate was 70.0％ (21/30).The concentrations of VEGF,Arg-1 and iNOS in test group before microwave ablation were (816.56 ± 13.26)pg/ml,(5.17±0.20) ng/ml and (544.18 ± 13.93)pg/ml,which were higher than those in control group (93.43 ± 9.93) pg/ml,(1.08 ± 0.05) ng/ml and (8.08 ± 0.33) pg/ml,and the differences were statistically significant (t =239.093,P ＜ 0.001;t =110.359,P ＜ 0.001;t =210.792,P ＜ 0.001).The concentrations of VEGF were (708.41 ± 10.49) pg/ml,(592.63 ± 7.25) pg/ml and (521.91 ± 8.32) pg/ml on the first day,third day and 1 month after microwave ablation in patients with advanced NSCLC,which were significantly lower than those before treatment (all P ＜ 0.05).The homologous concentrations of Arg-1 were (5.95 ± 0.10) ng/ml,(7.02 ± 0.13) ng/ml and (7.67 ± 0.92) ng/ml,which were significantly higher than those before treatment (all P ＜ 0.05).The homologous concentrations of iNOS were (453.01 ± 9.48) pg/ml,(393.21 ± 9.42) pg/ml and (352.60 ± 8.31) pg/ml,which were significantly lower than those before treatment (all P ＜ 0.05).The expression of iNOS was positively related with VEGF in NSCLC patients before treatment (r =0.379,P =0.039),and the expression of Arg-1 was negatively related with VEGF (r =-0.556,P =0.001).However,the expression of iNOS was not associated with Arg-1 (r =-0.238,P =0.205).Conclusion Microwave ablation is effective for local therapy of NSCLC,which can directly kill cancer cells,and affect the levels of VEGF,Arg-1 and iNOS.VEGF has certain correlation with iNOS and Arg-1,but there was no correlation between iNOS and Arg-1.Microwave ablation can change the tumor microenvironment in a certain extent,and stimulate the body to produce anti-tumor immunity.
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BACKGROUND:Our previous studies have demonstrated that melatonin inhibits the adipogenic differentiation of bone marrow mesenchymal stem cels. However, the mechanism underlying the effect of melatonin on adipogenesis is stil unknown. OBJECTIVE:To determine whether melatonin can inhibit the adipogenesis of bone marrow mesenchymal stem cels through retinoid-related orphan receptor α. METHODS:Bone marrow mesenchymal stem cels were isolated and purified by density gradient centrifugation combined with cellattachment culture. The phenotype was investigated by flow cytometry. Then, cels were induced for adipogenic differentiation with melatonin, CGP52608 and normal adipose tissue, respectively. The levels of retinoid-related orphan receptor α mRNA and protein were investigated by real-time RT-PCR and western blot assay, respectively. Further, the effect of retinoid-related orphan receptor α on the dipogenic differentiation of bone marrow mesenchymal stem cels was investigated by CGP52608. RESULTS AND CONCLUSION:The primary isolated bone marrow mesenchymal stem cels were spindle-shaped fibroblast-like cels. These cels did not express hematopoietic stem cels markers: CD34 and CD45; and highly expressed MSC markers: CD29, CD44, and CD10. The result of RT-PCR demonstrated that melatonin nuclear receptor, retinoid-related orphan receptor α, was highly expressed in bone marrow mesenchymal stem cels and the expression of retinoid-related orphan receptor α was further enhanced by melatonin in a dose-dependent manner, which was confirmed at protein level by western blot assay. During adiogenesis, the expression of retinoid-related orphan receptor αmRNA was up-regulated in the early stage, but maintained at a low level in the mild-later stage. While the retinoid-related orphan receptor α was activated by agonist CGP52608, the adipogenic differentiation of bone marrow mesenchymal stem cels was inhibited, which was similar to the inhibitory effect of melatonin. Therefore, melatonin inhibited the adipogenic differentiation of bone marrow mesenchymal stem cels through retinoid-related orphan receptor α, suggesting that melatonin plays an important role in the differentiation of adipocytes.
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Gastric cancer can spread to the liver through hematogenous metastasis,lymphatic metastasis and serosal invasion of primary tumor.The occurrence of gastric cancer with liver metastasis is only second to the occurrence of peritoneal metastasis.Liver metastasis is one of the main distant metastasis of gastric cancer,and is a major cause of cancer-related death.The treatment of gastric cancer with liver metastasis still remains controversial.The optimal treatment strategies should be based on the clinicopathological characteristics of each patients and evidence-based medicine,and the individualized plan should be set through multidisciplinary team discussions.Before the more results of prospective studies released,multidisciplinary treatment is the main method,and the appropriate patients should be cautiously choosed for surgery.
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Objective To detect the change of precursor protein of sol in breast cancer before and after the neoadjuvant chemotherapy,so as to find the serum protein marker of chemotherapy drug resistance in breast cancer.Methods Breast cancer patients with Ⅲ period neoadjuvant chemotherapy were enrolled in the study.The serum specimens of the patients were obtained.Two-way gel electrophoresis was used to get the gel map before and after chemotherapy,then the image map was analyzed by gel analysis software.The differentially expressed proteins point was screened before and after chemotherapy.Serum protein differences were identified with matrix auxiliary laser disi ntegrate series time of flight mass spectrometer (MALDI-TOF-MS),including coagulation sol precursor protein.Western blot was used to test the variation trend of them before and after chemotherapy.Results Coagulation sol precursor protein levels fell after neoadjuvant chemotherapy in resistance group.The variation trend of the results of western blot and that of the proteomics were consistent.Conclusion Coagulation sol precursor protein can be as the candidate serum marker of chemotherapy drug resistance in breast cancer.
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Objective To make clear of the mechanism of metastasis and recurrence of primary liver cancer, and to make sure of the preventive value of transeatheter arterial immun- ehemoembolization (ICE) in treatment of liver cancer. Methods The study is a double-blinded, controlled, prospective, randomized trial. 146 patients were randomly divided into 3 groups. Group A: regular surgery of primary liver cancer;group B: TACE 1month after surgery, treatment proposal was: ADM + DDP + 5-Fu + LP; group C:transeatheter arterial immun-chemoembolization 1 month after surgery, treatment proposal : ADM + DDP +5-Fu+LP+Octreotide+IFN. Results CD3+, CD4+, CD4+/CD8+ increased 1 month after surgery in group A; In group B: CD3+, CD4+, CD4+/CD8+ decreased but CD8+ increased 1 week after TACE; CD3+, CD4+, CD4+ /CD8+ increased but CD8+ decreased 4 weeks after TACE(P<0.05); In group C: CD3+ , CD4+, CD4+/CD8+ increased slightly 1 week after TACE, but increased significantly 4 weeks later(P<0.05). In group A, the level of VEGF was high before surgery but decreased 1 month after surgery; In group B, the level of VEGF decreased 1 week after TACE, decreased more significantly 4 weeks after TACE than that in group B(P <0.05). 1 year survival rate, 2 year survival rate and 3 year survival rate in group C ware higher than those in group A; 1 year survival rate was higher in group B than that in group A, however, 2 year survival had and 3 year survival rate had no differ-ences between group B and group A. Conclusion Transcatheter arterial ICE can prevent metastasis and recurrence after surgery of primary liver cancer increase the 1,2,3 year survival rate. It not only has the role of TACE,but can increase the immunefunction,and inhibit tumor angiogenesis, then increase the therapeutic effect.
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Objective To investigate the role of the pulmonary function, arterial blood gas analysis, the St George respiratory questionnaire (SGRQ) score and nutritional status in evaluating the development of chronic obstructive pulmonary disease (COPD). Method Changes of the above-mentioned parameters were analyzed in hospitalized patients suffered from moderate to severe COPD and compared with parameters of those who had acute exacerbations when admitted to hospital. Results The forced expiratory volume in one second (FEV1)% pred and oxygenation index of the patients declined significantly with the development of the disease after one, two, three, four times in hospital [(68.43 ± 3.09)%, (61.27 ± 3.38 )%, (42.05 ± 4.16)%, (33.64 ± 3.34)% and 435.55 ± 10.23, 404.35 ± 11.56, 358.38 ± 13.21, 321.29 ± 11.78] (P < 0.05), while the level of PaCO2 increased significantly in moderate COPD [(36.23 ± 3.62), (45.44 ± 4.67), (57.82 ± 4.12), (78.28 ± 5.21 )mm Hg (1 mm Hg = 0.133 kPa) ] (P<0.05). The oxygenation index was declined significantly in severe COPD. The scores of SGRQ score declined significantly in all patients while the level of hemoglobin, albumin and body mass index did not change significantly. Conclusions Acute ex-acerbation attributes to the deterioration of pulmonary function and life quality of COPD patients. Monitoring the changes of pulmonary function, arterial blood gas analysis and SGRQ score may be useful in the evaluation of the development of COPD.
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OBJECTIVE: To establish a HPLC assay for determining cassiaside B in rabbit plasma .METHODS: The proteinin sample was first denatured with methanol .The ?-- Bondapak C_(18) colurnn(3.9mm?300mm, 10?m) was used with a mobilephase of acetonitrile -- water -- THF -- glacial acetic acid(20:76.5:3.0:0.5) .The flow rate was 1.0ml/ min .Detecting wave-length was 278nm .RESULTS: The caliblation curve of cassiaside B was C=9.102?10~(-2)+4.871?10~(-5)R(r=0.9999) .Theaverage recovery of cassiaside B in plasma was (100.8?1.139)% .The relative standard deviations of intra -- day and inter -- dayassay were less than 2.08% .The detection limit in plasma was 0.05?g/ml .CONCLUSION: The method is sensitive and accu-rate. It is suitable for the pharmacokinetic study of cassiaside B.
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OBJECTIVE:To investigate the way of implementing quantized assessme nt in department of pharmacy METHODS:To set up the general quantized indexes of pharmacy and splitting indexes of various sections RESULTS:By way of quantize d assessment,the enthusiasm of staff members was aroused and the quality of pha rmaceutical service improved CONCLUSION:Implementing quantized assessment make s the realization of scientific management of pharmacy